Study on Complete Antigen Synthesis of Chlorpromazine

Abstract

Chlorpromazine (CPZ) is a small molecule, which cannot cause an immune response in the body and does not have active groups such as the amino carboxyl group. Therefore, the molecular structure of CPZ needs to be modified to introduce active groups, so that it can be conjugated with a carrier protein to form a complete antigen to induce an immune response and produce antibodies. In this paper, the molecular structure of chlorpromazine is modified by the chemical synthesis method. The diazotization of p-aminobenzoic acid into diazo salt by sodium nitrite is reacted with chlorpromazine. The aromatic side ring containing the carboxyl group is introduced into the counterposition of chlorpromazine. Artificial complete antigen CPZ-BSA is synthesized by mixed anhydride method catalyzed by triethylamine and isobutyl chloroformate and bovine serum albumin (BSA). The complete antigen is identified by ultraviolet spectroscopy. Results The characteristic absorption peaks of the synthetic complete antigen are not in the same position as those of chlorpromazine and bovine serum albumin, and the conjugation of chlorpromazine and bovine serum albumin is confirmed to be successful. The complete antigen of chlorpromazine is synthesized, which lays a foundation for the further determination of chlorpromazine residues, the establishment of immunological detection methods and the preparation of antibodies.