Abstract

The motive of this work was to provide an inexpensive potential wound dressing using chitosan lactate (LCH) which was synthesized by the grafting lactic acid onto the amino groups in chitosan (CH) without a catalyst. The XRD and 13CNMR results demonstrated that the grafting by lactic acid took place at C2 site in CH, leading to the destruction of the regularity of the packing in the original CH chains and formation of the amorphous CH salts. The unique device was developed in our experiments which could yield an approximately vertical thermal gradient, forming the uniformly vertical pores in LCH sponges. TEM images revealed that both TBA and LCH concentration affected the micro-structure of the sponges, although they worked via different mechanisms. In the water suction experiments, the capillary coefficient Ks was introduced to evaluate the structure-function relationship. The positive or negative influence of LCH, TBA and porosity on Ks clearly stood out when their relationships were plotted graphically. The in vitro biocompatibility of LCH sponges was evaluated. The results obtained indicated that LCH sponges exhibited bio-safety at lower concentration (25%) during short time (1 day). However, highly concentrated extraction showed a serious toxic effect on both HSF and HaCaT cells. The release kinetics for hydrophilic and hydrophobic drugs with different formulation sponges was determined in in vitro release experiments. The contribution of the drug diffusion, matrix erosion and microstructure of porous materials must be taken into account on the release mechanism. The method and the structure described in present paper provided a starting point for the design and fabrication of a family of chitosan derivatives based porous materials with potentially broad applicability

Highlights

  • Chitosan is obtained from the partial deacetylation of chitin which is the main component of the exoskeleton of crustaceans, composing of 2-amino-2-deoxy-D-glucose units linked through β-(1→4) bonds

  • The results suggest that the higher concentration extraction medium from LCH sponges is harmful for the Human skin fibroblasts (HSF) cells

  • Due to the presence of two functional groups chitosan was modified by lactic acid without using catalysts in our present experiments

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Summary

Introduction

Chitosan is obtained from the partial deacetylation of chitin which is the main component of the exoskeleton of crustaceans, composing of 2-amino-2-deoxy-D-glucose units linked through β-(1→4) bonds. It is crystalline and shows polymorphism depending on its physical state. Numerous works have been done on the modification of chitosan, leading to various derivatives with improved properties. Among these derivatives, chitosan lactate (LCH) is very popular for its great biocompatibility, antibacteria, low toxicity and simple preparation. Controlling the structure of LCH can be done by drying processes in suitable molds.

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