Study on Chemical Forms of Iron in Photographic Gelatin†

Abstract

AbstractThe combination of a protein separation technique and atomic absorption analysis was utilized to study chemical forms of iron in photographic gelatins. Gel filtration with Sephadex G-150 gel and a TR1S—chloride buffer was used for separating proteins in photographic gelatin. Using the flameless atomic absorption spectrum method, the distribution of iron in photographic gelatin was found with respect to molecular weight, indicating major binding to protein. Iron free from protein was also found in small amounts. Addition of inorganic iron caused an increase of free iron content in photographic gelatin.