Abstract
The objective of this study is to determine the erianin of 10 species of Dendrobium by high performance liquid chromatography (HPLC). Processing techniques were washed, dried and ground. The research method for determining the content of erianin was adopted from Chinese Pharmacopoeia 2015 Edition. Acetonitrile-0.05% phosphoric acid (37:63) was used as mobile phase. The samples were separated on Sharpsil-TC18 column(4.6*150mm;5μm) at a flow rate of 1.2mL/min and detected at 230nm, and the column temperature was kept at 30℃. The injection volume was 20μL. The number of theoretical plates was not less than 6000 according to the chromatographic peak of erianin. The result of the study showed erianin was detected only in Dendrobium chrysotoxum among the 10 species of Dendrobium. and the content was 0.098%. Conclusion: The content of erianin in Dendrobium chrysotoxum met the specification of no less than 0.03% in the 2015 edition of Chinese Pharmacopoeia.
Highlights
Dendrobium is one of the richest genus of Orchidaceae with more than 1100 species widely distributed in Asian, Europe and Australia
There are 74 species and 2 variations of Dendrobium found in China which are mainly distributed in Yunnan, Sichuan and Guangxi provinces [1]
Wild Dendrobium resources have been over-excavated, making wild Dendrobium endangered in recent years
Summary
Dendrobium is one of the richest genus of Orchidaceae with more than 1100 species widely distributed in Asian, Europe and Australia. There are 74 species and 2 variations of Dendrobium found in China which are mainly distributed in Yunnan, Sichuan and Guangxi provinces [1]. Dendrobium is one of the most well-known orchids in global horticultural trade due to its beautiful flowers and ideal characteristics as houseplants. Dendrobium has been used as traditional herbal in many Asian countries for a long time. Wild Dendrobium resources have been over-excavated, making wild Dendrobium endangered in recent years. As a traditional medicinal and ornamental plant in
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