Abstract

Sea water joining diesel was selected to prepare simulated marine oil pollution. With the target of removing diesel from seawater, the influence of various factors on the laccase-catalyzed degradation process was discussed. The experimental results show that the laccase-catalyzed degradation process was significantly affected by laccase dosage, reaction temperature, reaction time, pH of the solution and initial concentration of diesel in the oily wastewater. A systematic optimization study was carried out through a orthogonal test on the basis of the results of the single-factor experiments,and the optimum reaction conditions of laccase catalytic degradation diesel pollutants in seawater was determined. The results indicate that under the conditions of diesel initial concentration of 0.1g/L, laccase dosage of 8mg/L, pH value of 6, the reaction temperature of 25°C and the reaction time of 4h, laccase catalytic degradation rate of diesel pollution can be up to 63.85%.

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