Abstract
In this work, the soil samples were collected niche habitats of Valparai hill station to screen the diversity of actinomycetes. The actinobacterial were isolated by serial dilution and plating method on starch peptone agar media. In totally 8 different morphological were isolated on the basis of colony characteristics on starch peptone agar and dominative isolate were screened and plated on point inoculation. RRMVCBNR 1 isolate was biosystamatically characterized on the basis of microscopic, colony morphology, biochemical and phenotypic studies. Phenotypic studies indicated that strains belonged to genus of Nocardiopsis which was further confirmed by genotypic studies based on 16S rRNA gene sequences followed by phylogenetic tree construction. 16S rRNA gene sequences of strain used in this study exhibited sequence similarity in the range of 99-100% with those of selected isolate and it was identified as of Nocardiopsis flavescencs RRMVCBNR. The sequences of Nocardiopsis flavescencs 16S rRNA genes were deposited in genbank http://www.ncbi.nlm.nih.gov/genbank and received the accession number MG287120.The biological activity of Nocardiopsis flavescencs strain showed detectable antibacterial activity against Staphylococcus aureus and Klebsiella pneumonia has been studied. Keywords: Nocardiopsis flavescencs, Starch peptone agar, Muller Hinton Agar, Mountain soil.SEM
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