Study on Association of Single Nucleotide Polymorphism of MC3R and MC4R Genes with Carcass and Meat Quality Traits in Chicken

Abstract

Body composition, fat deposition and meat quality are important traits in chickens. Melanocortin receptor (MCR) plays an important role in central melanocortin system (CMS) and muscle cells. The purpose of the present study was to analyze association of the MC3R and the MC4R genes with chicken carcass and meat quality traits. Using eight meat-type chicken populations constructed with 5 pure lines (developed from Chinese local breeds) and 3 crossbreeds (S01×D99, S01×S05, S01×S10), the association of 3 single nucleotide polymorphisms (SNP: MC3R-A1424G, MC4R-G923T and MC4R-C944T) of MC3R and MC4R gene with carcass and meat quality traits was studied. The results showed as follows: (1) the MC3R-A1424G genotypes were significantly associated with most carcass traits except for semi-eviscerated percentage and leg muscle percentage (LMP), the MC4R-G923T genotypes were significantly associated with live weight, carcass weight, leg muscle weight (LMW) and LMP, and the MC4R-C944T genotypes were not significantly associated with most carcass traits except for LMW and LMP; (2) to meat quality, the MC3R-A1424G genotypes significantly affected muscle crude protein (GP) value, and the allele A had positive additive effects on slaughter traits. The MC4R-G923T and the MC4R-C944T sites significantly affected muscle GP value and glutamic acid (Glu) value; (3) the haplotypes based on the 2 SNP of MC4R gene were also significantly associated with meat quality traits, but had no significant associations with carcass traits. The research built the base for further analysis on relation between genetic variation of MC3R and MC4R genes and the carcass and meat quality traits, and molecular marker's application in breeding.