Study on Applicability of Sucrose Ester in Tissue Culture Media

Abstract

The main purpose of this study was to find out applicability of sucrose ester in tissue culture media. Sucrose ester is classified as an emulsifier and it is usually used to improve texture, aeration, protein protection and sugar crystallization. SEs is used in many applications including bakery, confectionery, cereals, dairy, ice cream and sauces. Murashige and Skoog medium supplemented with BAP (3 mg/l) and IAA (0.5 mg/l) was used for tissue culture of gerbera. The nutrient mediums were supplemented with different concentrations of sucrose esters (0 to 0.5%). Shoot formation was highly influenced by concentrations of sucrose ester in medium. Among different concentrations for shoot multiplication, best performance was showed on medium supplemented with 0.025% sucrose ester. Average number of shoots per explants was higher on this medium (9.58±) with average height of longest shoot 5.05 ± 0.02. However, the number of shoots and height of shoot was significantly reduced when SE concentration in media was further increased (0.05 to 0.5%). This indicates that we can use sucrose ester in tissue culture media for improving growth of tissue cultured plantlets (number of shoots and height of shoots) and upper limit for use of SE in tissue culture media is 0.025%.