Abstract
Objective. To investigate the synergistic effects of IκBα overexpression and poly (lactic-co-glycolic acid)-curcumin nanoparticles (PLGA-Cur-NPs) on prostate cancer (PC) and reveal the underlying mechanisms of cooperative sensitization induced by curcumin. Methods. Proliferation and apoptosis rate in IκBα overexpressed and PLGA-Cur-NPs-treated PC cells were detected by MTT and flow cytometry assay. The expression levels of IκBα, apoptosis-related, and signaling proteins were measured by western blotting assay. Results. PC cell proliferation was significantly inhibited by the overexpression of IκBα. The apoptosis rate of PC cells was significantly increased at a high concentration of curcumin exposure, while the activation of NF-κB pathway was obviously inhibited. In addition, PLGA-Cur-NPs treatment synergistic with IκBα overexpression enhanced the apoptosis of PC cells by suppressing the NF-κB pathway activation. Conclusion. IκBα overexpression synergistic with PLGA-Cur-NPs could obviously inhibit proliferation, induce apoptosis, and suppress the activation of NF-κB pathway in PC cells. These findings may provide an experimental basis to establish the tumor gene therapy combined with traditional Chinese medicine treatment, thus promoting the clinical application of both tumor gene therapy and anti-tumor Chinese medicine.
Highlights
IntroductionThe incidence of prostate cancer (PC) has been gradually increasing. It is worth noting that the clinical treatment of advanced PC faces the challenge of androgen resistance in PC cells, which will lead to the loss of antiandrogen therapy. erefore, it is extremely urgent to develop new treatments [1,2,3]
Up to now, the incidence of prostate cancer (PC) has been gradually increasing
It is worth noting that the clinical treatment of advanced PC faces the challenge of androgen resistance in PC cells, which will lead to the loss of antiandrogen therapy. erefore, it is extremely urgent to develop new treatments [1,2,3]
Summary
The incidence of prostate cancer (PC) has been gradually increasing. It is worth noting that the clinical treatment of advanced PC faces the challenge of androgen resistance in PC cells, which will lead to the loss of antiandrogen therapy. erefore, it is extremely urgent to develop new treatments [1,2,3]. Erefore, it is extremely urgent to develop new treatments [1,2,3]. Cationic poly lactic-co-glycolic acid nanoparticles (PLGA-NPs) are a new particle coated with a cationic surfactant and charge to reconcile agent gene nanoparticles. Multiple studies showed the advantages of cationic PLGA-NP carrier in aspects of genetic, nucleic acid protection, transfection efficiency, and target gene expression. As a new type of drug carrier, PLGA-NPs could improve the water solubility of drugs and control the drug release rate by encapsulating drugs, increasing drugs’ bioavailability, and reducing adverse drug reactions [4,5,6,7,8]. PLGA-NPs have been approved as carriers to carry drugs for human experimental research due to their biodegradable and nontoxicity
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