Study on Apoptosis of NCI-H460 Cells Induced by Chloroform Extract of Leaves from Crinum Asiaticum

Abstract

Objective To investigate the effect of chloroform extracts(CE) of leaves from Crinum asiaticum on proliferation and apoptosis of non-small cell lung cancer(NSCLC) NCI-H460 cells.Methods The activity of cell growth inhibition of NCI-H460 cells was measured by MTT assay.Morphological changes of cell nuclei were observed by Hoechst 33258 staining.The expression levels of Bcl-2 and Bax proteins were examined by immunocytochemistry staining.Flow cytometry was performed to measure the cell cycle.Results CE displayed growth inhibitory activity against NCI-H460 cells with IC50 values of 36.22 ±3.04,41.21±2.50 and 62.55±3.47 mg/L for 24,48 and 72 h,respectively.The effect enhanced with an increase in the concentration of CE.The condensed chromatin,nuclear fragmentation and a large number of apoptotic bodies was observed by Hoechst 33258 staining.The percentage of apoptosis in NCI-H460 cells increased after treated with CE,compared with control.The expression level of Bax protein in NCI-H460 cells cultured with CE were higher than that of control,and the expression level of Bcl-2 protein in NCI-H460 cells dealt with CE were lower than that of control.The arrest of NCI-H460 cells in G1/S phases was induced by CE.Conclusion In vitro CE significantly inhibited the growth of NCI-H460 cells.Antitumor mechanism of NCI-H460 cells may be associated with the changes of cell cycle and cell apoptosis.