Abstract
PSORI-CM02 is an optimization formula of PSORI-CM01, which is a clinical herbal formula for the treatment for psoriasis in the Guangdong Provincial Hospital of Chinese Medicine. Previous research indicates that it plays a critical role in anti-inflammation and immunoregulation. Rhizoma smilacis glabrae (RSG) is one herbal medicine of PSORI-CM02, whose effective anti-inflammatory component is astilbin. This study aims to test the anti-inflammatory and immunoregulation effects of astilbin as well as RSG in PSORI-CM02, and we, respectively, used the CuSO4-induced neutrophil-specific transgenic zebrafish model Tg(mpx: EGFP) and the melanin allele mutated Albino strain zebrafish model to visualize the effects of neutrophil recruitment and macrophage phagocytosis. Our data indicated that both PSORI-CM02 and astilbin had anti-inflammatory effects, leading to a reduction in the recruitment of neutrophils and promotion in macrophage phagocytosis. Nevertheless, the negative liquor of Rhizoma smilacis glabrae (PSORI-CM02 without RSG) also had anti-inflammatory and promoting macrophage phagocytosis effects. The results revealed the formula excluding RSG also had anti-inflammatory and immunoregulation effects, which demonstrated that RSG was not the major anti-inflammatory herbal medicine in PSORI-CM02. Similarly, astilbin was not the major anti-inflammatory active ingredient in the formula. The anti-inflammatory and the promotion of macrophage phagocytosis effect of PSORI-CM02 in vivo zebrafish were the results of multiple component interaction, which was the common characteristic of the Chinese medicine compound.
Highlights
Inflammation is a complicated biological and pathophysiological cascade of responses to infections and injuries
Inflammatory mechanisms are closely linked with many diseases including cardiovascular disease, rheumatoid arthritis, inflammatory bowel disease, Alzheimer’s disease, and even cancer [1, 2]. e inflammatory process is mediated by diverse inflammatory cells, including neutrophils, macrophages, eosinophils, and mononuclear phagocytes [3]
Phagocytosis of pathogens, apoptotic cells, and debris is a critical feature of macrophage function in host defense and tissue homeostasis [8]. erefore, monitoring the number of neutrophils in animals and examining macrophage phagocytosis are effective methods for evaluating inflammatory responses. e occurrence and development of many diseases are related to immunity and inflammation
Summary
Inflammation is a complicated biological and pathophysiological cascade of responses to infections and injuries. RSG, which is a traditional Chinese medicine in PSORICM02, has been reported to have an anti-inflammatory effect, and its major effective active component is astilbin [16]. We sought to clarify whether RSG is the main traditional Chinese medicine that has an anti-inflammatory role and astilbin is the main active component of anti-inflammation in PSORI-CM02. To this end, two pathological models of zebrafish were established: the copper sulfate-induced zebrafish inflammation model and the zebrafish macrophage phagocytosis function evaluation model. Related pharmacodynamic experiments were conducted to evaluate the anti-inflammatory effect and correlation among astilbin, RSG, and PSORI-CM02
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
