Study on antibacterial activity of silver nanoparticles synthesized by gamma irradiation method using different stabilizers.

Dang Van Phu,Nguyen Thi Kim Lan,Nguyen Ngoc Duy,Nguyen Quoc Hien,Le Anh Quoc,Le Quang Luan,Bui Duy Du

doi:10.1186/1556-276x-9-162

Abstract

Colloidal solutions of silver nanoparticles (AgNPs) were synthesized by gamma Co-60 irradiation using different stabilizers, namely polyvinyl pyrrolidone (PVP), polyvinyl alcohol (PVA), alginate, and sericin. The particle size measured from TEM images was 4.3, 6.1, 7.6, and 10.2 nm for AgNPs/PVP, AgNPs/PVA, AgNPs/alginate, and AgNPs/sericin, respectively. The influence of different stabilizers on the antibacterial activity of AgNPs was investigated. Results showed that AgNPs/alginate exhibited the highest antibacterial activity against Escherichia coli (E. coli) among the as-synthesized AgNPs. Handwash solution has been prepared using Na lauryl sulfate as surfactant, hydroxyethyl cellulose as binder, and 15 mg/L of AgNPs/alginate as antimicrobial agent. The obtained results on the antibacterial test of handwash for the dilution to 3 mg AgNPs/L showed that the antibacterial efficiency against E. coli was of 74.6%, 89.8%, and 99.0% for the contacted time of 1, 3, and 5 min, respectively. Thus, due to the biocompatibility of alginate extracted from seaweed and highly antimicrobial activity of AgNPs synthesized by gamma Co-60 irradiation, AgNPs/alginate is promising to use as an antimicrobial agent in biomedicine, cosmetic, and in other fields.

Highlights

The common stabilizers used in bottom-up approach of metallic nanoparticle synthesis (Au, Pt, Ag, etc.) are polymers and surfactants such as sodium dodecyl sulfate (SDS) and Tween 80 [1]
The study results of El Badawy et al showed that AgNPs/polyvinyl pyrrolidone (PVP) had the higher antibacterial activity than AgNPs/citrate and especially AgNPs capped by branched polyethyleneimine (BPEI) had better antibacterial activity than the other ones [20]
It is obvious that the stabilizers affected the size of AgNPs synthesized by the gamma Co-60 irradiation method

Summary

Introduction

The common stabilizers used in bottom-up approach of metallic nanoparticle synthesis (Au, Pt, Ag, etc.) are polymers and surfactants such as sodium dodecyl sulfate (SDS) and Tween 80 [1]. The study results of Kvítek et al showed that the antibacterial activity of silver nanoparticles (AgNPs) was significantly enhanced by using the suitable stabilizers such as the following surfactants: SDS, Tween 80, and PVP K90 [1]. The study results of El Badawy et al showed that AgNPs/PVP had the higher antibacterial activity than AgNPs/citrate and especially AgNPs capped by branched polyethyleneimine (BPEI) had better antibacterial activity than the other ones [20]. They claimed that the mechanism of AgNP toxicity may involve a combination of both physical and chemical interactions. The various stabilizers for AgNPs affect on the stability and on the antibacterial activity of AgNP colloid [1,14,20,21]

Methods

Results

Conclusion