Abstract
Chinese oak tasar Antheraea pernyi silk fibroin nanoparticles are a promising biomaterial for drug delivery because of its good properties, such as biodegradability and biocompatibility. In recent decades, self-assembling nanoparticles derived from Antheraea perny silk fibroin have risen too much interest in application of drug delivery. In this paper, the Ca2+ in calcium gluconate induced self-assembling nanoparticles. It was fabricated by blending aqueous Antheraea perny silk fibroin solution, calcium ion and insulin in a proper mixing ratio. The structure of the silk nanoparticles carried insulin was characterized by X-ray diffraction, FTIR and DTA. The size and the morphology of silk nanoparticles were tested using scanning electron microscope and particulate size description analysers. The drug content, covering rate and in vitro cumulative release of silk nanoparticles were also determinate. The results demonstrated that the Ca2+ induced selfassembling nanoparticles hold the particle size of 200~500 nm. The phenomenon of the sustained release is comparatively obvious in the silk nanoparticles. To summarize, this research will open up a new experimental basis on the usage of macromolecular drug, insulin which can properly meet the satisfaction of the patients desired for sustained-release insulin.
Highlights
The gradual improvement of the living standard of human beings, the incidence of diabetes is increasing day by day
Factory), Chinese oak tasar Antheraea perny silk fibroin (AR, ASF nanoparticles were distributed in water by ultra-sonication, ShangHai jin Lu Trade Co., Ltd.), ammonium thiocyanate
The utilization ratio of ASF in ASF-INS nanoparticles changed linearly with the mass ratio of INS to ASF. When it is fixed the concentration of silk fibroin protein at 20 mg/ mL, it is seen that the utilization of ASF has a gradual downward trend from 98% to 88% with the increasing amount of INS
Summary
The gradual improvement of the living standard of human beings, the incidence of diabetes is increasing day by day. Diabetes does not have the obvious symptoms of cardiovascular disease, the horror of AIDS and the intense rapid of cancer. Diabetes acts an important role quietly among several major "killers" who endanger human life. Diabetes becomes the third largest diseases killer after cardiovascular diseases and cancer in threating to human health. Silk fibroin has polypeptide chain segments, which presents random curl in dilute solution, α-helical structures in concentrated solution and water-insoluble β-hairpin formed while silkworms spin silk [1,2]. Silk fibroin has good biocompatibility, biodegradability, and non-toxicity so that it can become a promising application of drug delivery medical materials
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
