Abstract
Neural stem cells (NSCs) exist in the central nervous system of adult animals and capable of self-replication. NSCs have two basic functions, namely the proliferation ability and the potential for multi-directional differentiation. In this study, based on the bioassay-guided fractionation, we aim to screen active components in Cuscuta chinensis to promote the proliferation of NSCs. CCK-8 assays were used as an active detection method to track the active components. On the basis of isolating active fraction and monomer compounds, the structures of these were identified by LC-MS and (1H, 13C) NMR. Moreover, active components were verified by pharmacodynamics and network pharmacology. The system solvent extraction method combined with the traditional isolation method were used to ensure that the fraction TSZE-EA-G6 of Cuscuta chinensis exhibited the highest activity. Seven chemical components were identified from the TSZE-EA-G6 fraction by UPLC-QE-Orbitrap-MS technology, which were 4-O-p-coumarinic acid, chlorogenic acid, 5-O-p-coumarinic acid, hyperoside, astragalin, isochlorogenic acid C, and quercetin-3-O-galactose-7-O-glucoside. Using different chromatographic techniques, five compounds were isolated in TSZE-EA-G6 and identified as kaempferol, kaempferol-3-O-glucoside (astragalin), quercetin-3-O-galactoside (hyperoside), chlorogenic acid, and sucrose. The activity study of these five compounds showed that the proliferation rate of kaempferol had the highest effects; at a certain concentration (25 μg/mL, 3.12 μg/mL), the proliferation rate could reach 87.44% and 59.59%, respectively. Furthermore, research results using network pharmacology techniques verified that kaempferol had an activity of promoting NSCs proliferation and the activity of flavonoid aglycones might be greater than that of flavonoid glycosides. In conclusion, this research shows that kaempferol is the active component in Cuscuta chinensis to promote the proliferation of NSCs.
Highlights
Ischemic stroke (IS), an acute cerebral blood circulation disorder, often occurs in the elderly
We found that kidney-tonifying traditional Chinses medicine Velvet Antler, Cuscuta chinensis, Rehmannia glutinosa, Achyranthes bidentata, Lycium barbarum, Morinda officinalis, Cistanche deserticola, Polygonatum sibiricum, Epimedii Folium, Cornus officinalis, and Polygoni Multiflori had the activity of promoting Neural stem cells (NSCs) proliferation, among which Velvet Antler showed the highest activity, followed by Cuscuta chinensis
The objective of this study was to screen active components in Cuscuta chinensis to promote the proliferation of NSCs, which contribute to the discovery of related drugs with clinical application potential
Summary
Ischemic stroke (IS), an acute cerebral blood circulation disorder, often occurs in the elderly. Due to most IS patients unable to effectively receive treatment in the acute phase, they may inevitably enter the stroke sequelae stage. Stroke sequelae mainly refers to a condition left after hemorrhage of acute cerebrovascular disease, hemiplegia, numbness, skewed eyes, and poor speech [2,3]. The treatment of stroke sequelae focuses on rehabilitation, lacking effective treatment methods. Some reports have shown that traditional Chinese medicine (TCM) plays an important role in the treatment of stroke sequelae [4,5]. In the case of stroke sequelae patients, it is necessary to find effective natural products in TCM to promote nerve recovery
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