Abstract
We have synthesized a tritiated form of 2,22-dideoxyecdysone ((3H2-22,23)-3β,14α, 25-trihydroxy-5β-cholest-7-en-6-one) of high specific activity (2.2 TBq/mmol). We have examined the capacity of various endocrine (prothoracic glands, follicle cells) and peripheral (fat body, Malpighian tubules) tissues of Locusta migratoria to use this molecule as a precursor of ecdysone biosynthesis. Efficient conversion of 2,22-dideoxyecdysone to 2-deoxyecdysone and to ecdysone could principally be monitored in the prothoracic glands and follicle cells.
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