Abstract
A possibility of the creation of potentiometric biosensor by adsorption of enzyme urease on zeolite was investigated. Several variants of zeolites (nano beta, calcinated nano beta, silicalite, and nano L) were chosen for experiments. The surface of pH-sensitive field-effect transistors was modified with particles of zeolites, and then the enzyme was adsorbed. As a control, we used the method of enzyme immobilization in glutaraldehyde vapour (without zeolites). It was shown that all used zeolites can serve as adsorbents (with different effectiveness). The biosensors obtained by urease adsorption on zeolites were characterized by good analytical parameters (signal reproducibility, linear range, detection limit and the minimal drift factor of a baseline). In this work, it was shown that modification of the surface of pH-sensitive field-effect transistors with zeolites can improve some characteristics of biosensors.
Highlights
Zeolites are microporous crystalline solids with welldefined structures
We studied the method of urease adsorption on the surface of pH-sensitive field-effect transistors (ISFET) using monolayer of different types of zeolites: silicalite, nano beta zeolite, zeolite nano L and calcinated nano beta zeolite
We investigated a possibility of application of a new method of immobilization of enzyme urease on the surface of pH-sensitive field-effect transistors by adsorption on zeolite monolayers
Summary
Zeolites are microporous crystalline solids with welldefined structures. They contain silicon, aluminium and oxygen in their framework and cations, water and/or other molecules within their pores. Many of them occur naturally as minerals and are extensively mined in different parts of the world. A defining feature of zeolites is that their frameworks are made up of four connected networks of atoms. One can think about this in terms of tetrahedral, with a silicon atom in the middle and oxygen atoms at the corners. These tetrahedra can link together by their corners to form a rich variety of beautiful structures.
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