Abstract
Process of green crop fractionation (GCF) was considered and its byproducts were tested for evaluation. The byproducts obtained were pulp, LPC (Leaf protein concentrate), Juice, Deproteinised juice and pressed crop (PC). The dry weight of LPC from each crop was measured. Maize forage LPC was more as compared with other forages. Deproteinised Juice (DPJ) is utilised as a medium for the fermentation of yeast. This yeast growth was compared with the yeast grown on Hansens broth media as control. Various concentration ratios of DPJ and Hansens media were prepared and yeast was fermented. Yeast mycelia grown on DPJ was found higher as compared with other concentrations. The culture filtrates of yeast mycelia collected and used to study the enzyme invertase by immobilisation method and the presence of alcohol was by iodoform test. Almost all the samples of various concentrations showed the presence of alcohol by proper secretion of enzyme zymase. Enzyme invertase appropriately found less in the culture filtrate of yeast mycelia grown on DPJ alone. Therefore DPJ is responsible for less breakdown of sucrose as compared with Hansens media which breaks down more sucrose into glucose and fructose by the enzyme invertase.
Highlights
In leaf protein (LP) research, during the process of green crop fractionation (GCF), the pulp is obtained [1].This pulp is squeezed to obtain the juice
After 8 days, the mycelial Deproteinised juice (DPJ) is filtered by whatman filter paper no.1 and the culture filtrate obtained are collected and utilised for the study of enzyme invertase.The mycelia obtained on filter paper is dried along with filter paper is dried in hot air oven
When different concentratons of DPJ were utilised for the yeast fermentation and along with it when Hansens broth was used at different concentrations, there were different results
Summary
In leaf protein (LP) research, during the process of green crop fractionation (GCF), the pulp is obtained [1].This pulp is squeezed to obtain the juice. This juice is heated to 90°C to coagulate the proteins to form the precipitate. The residue obtained is called LPC (Leaf protein concentrate), as the byproduct. To avoid pollution because of its disposal, its proper usage is advisable It contains 70% of carbohydrates along with vitamins and minerals [4,5].This can be used as manure or growing different economically important fungi [6,7]. Fresh juice during previous investigation when used for fermentation, secretes hydrolytic enzymes and effects on the yields of LPC [9]
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