Abstract
Interaction of Alizarin Red-S dye with thiamin (vitaminB1) was studied using spectrophotometric method at room temperature and neutral pH. The yellowish color appeared due to the Alizarin Red-S interaction with vitamin B1. According to the Benesi-Hildebrand equation, the related equilibrium constant, Kb, for the interaction has been determined and the Gibbs free energy of interaction has been calculated. Also the Benesi-Hildebrand equation has been used for assay of vitamin B1 in tablets. Results show that the method has good sensitivity for vitamin B1 and is in good agreement with HPLC method.
Highlights
Thiamin (2-[3-[(4-amino-2-methyl-pyrimidin-5-yl) methyl]-4-methyl-thiazol-5-yl] ethanol), is a water soluble vitamin called vitamin B1 (Figure 1)
The binding constant was determined from the effect observed in the absorbance of Alizarin Red-S at 422 nm upon addition of the thiamin according with the Benesi-Hildebrand treatment [20]
Where CARS and CT are the analytical concentrations of Alizarin Red-S and Thiamin in solution, respectively
Summary
Thiamin (2-[3-[(4-amino-2-methyl-pyrimidin-5-yl) methyl]-4-methyl-thiazol-5-yl] ethanol), is a water soluble vitamin called vitamin B1 (Figure 1) It is a natural essential nutrient which is found in rice bran, nuts, banana, soybean, green peas and etc. Thiamin was first discovered in 1910 by Umetaro Suzuki in Japan when researching how rice bran cured patients of beriberi [1] It was first crystallized by Jansen and Donath in 1926 and its chemical composition and synthesis was reported by Robert R. Phosphate derivatives of thiamin play great roles as co-factor in enzymatic processes inside human body including carbohydrate metabolism and nerve transduction [4] It is used in the metabolism of branched chain amino acids and may have non coenzyme roles in excitable cells [5]. Beriberi is a chronic deficiency disease that results from inadequate dietary
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Journal of Applied Solution Chemistry and Modeling
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
