Abstract
Microbial volatile organic compounds (MVOCs) are present in several production processes in biotechnology. In this study it is evaluated the viability of use of a method involving solidphase microextraction (SPME), in vivo SPME, in the extraction of MVOCs, associated with the pigment production process by the Monascus fungus. PDMS/Car/DVB (50/30 μm) fiber in the headspace mode (HS-SPME) and gas chromatography-mass spectrometry (GC-MS) were used to fermentation broth study. Seventeen compounds were found associated with the presence of the fungus, being the main ones ethanol, 2-methyl-propanol, 3-methyl-butanol, 2-methyl-butanol and 2-phenyl-ethanol. These compounds have maximum concentration of 313.87, 5.51, 2.37, 0.95 and 0.70 mg L −1 . The values of biomass and red pigment found for fermentation for ten days were 4.03 ± 0.21 g L −1 and 3.96 ± 0.17 (UA480). Regular MVOC productions were observed in this study, which were useful in the prediction and control of fermentation by Monascus fungus.
Highlights
Recent researches have evaluated a number of volatile organic compounds produced by microorganisms called Microbial volatile organic compounds (MVOCs) in order to understand the relations and interactions of these compounds with the culture units and with other system microorganisms
The integrated area values, obtained from the total ion chromatograms, were used as responses to design because they represented the amounts of MVOC compounds present in the headspace of the samples of the fermentative liquid
The use of a method involving solidphase microextraction (SPME) is essential to obtain results with greater reliability when evaluating the profiles of MVOCs during a fermentation process
Summary
Recent researches have evaluated a number of volatile organic compounds produced by microorganisms called MVOCs in order to understand the relations and interactions of these compounds with the culture units and with other system microorganisms. Flexibility to adapt to several analytes of biochemical interest, including some associated with metabolome.[8] Compounds which are product of biosynthesis, such as alcohols, ketones, esters, and compounds containing sulfur were successfully studied in living organisms through this technique.[5] In this study it is evaluated the viability of use of a method involving solid-phase microextraction, in vivo SPME, in the extraction of MVOCs, associated with the pigment production process by the Monascus fungus. The evaluated compounds are emitted or produced during the fermentation process and after the end of the process It was used a benchtop fermenter especially projected for withdrawing fermentation liquid samples in real time, with the best efficiency in material and biological resources use.[3] Some studies use solid-phase microextraction to evaluate volatile organic compounds of biological materials, mainly targeting the pharmacological and toxicological areas.[9,10] Few studies with emphasis on biotechnology and food area are found, where solid-phase microextraction is used to evaluate metabolites during the fermentation process (in line). The biomass was measured on a CP2P balance (Sartorius, Goettingen, Germany), with 0.001 mg precision
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