Study of Variability at the Molecular Level Between Pure Lines of Cherry Tomato

Abstract

Executed experience to study molecular variation and to determine the genetic fingerprint and Genetic dimension between the pure lines of a tomato Cherry are: LA3002(1),LA2838A(2),LA0797(3),LA4355(4),LA7371(5),LA3534(6),LA3535(7) and L.A. 3530(8) based on genetic indicators (ISSR and SSR) using 14 starters including 9 primers ISSR and 5 beginnings SSR and it was total package produced from these prefixes (106) package, at the rate of (7.57) packages approximately per starter, and it was the sum of the packages dissimilar in the primers shown (64) strap and represented its ratio (60,377% of the total packages that resulted at rate approximately (5) packages for the initiator. Some of the primers used showed distinct bands (visible or absent) for some pure lines that acted as genetic fingerprint for them, such as pure lines 2, 3, 6 and 8. The results showed that the highest genetic similarity among the pure lines was (0.989) between the two pure lines (4) (LA4355) and (LA7371) (5). We conclude from these results it isn’t possible to use the pointers genetics of DNA for both types (ISSR and SSR) in finding the genetic signature of pure lines of cherry tomato and its classification ame different groups and estimate the genetic distance between them for use in future education programs.