Abstract
目的 使用α-氰基丙烯酸烷基酯建立人肝癌-裸鼠皮下肝原位移植瘤模型.方法将BEL-7402人肝癌细胞接种于裸鼠皮下,建立皮下模型.形成皮下移植瘤后再移植于裸鼠肝内,利用α-氰基丙烯酸烷基酯建立肝原位移植瘤模型(间接肝原位移植瘤模型).结果成功地利用α-氰基丙烯酸烷基酯建立了间接肝原位移植瘤模型.移植瘤存活率达95.8%.结论与传统的肝原位移植瘤模型比较,利用α-氰基丙烯酸烷基酯建立间接肝原位移植瘤模型方法简便,成功率高,便于推广使用。
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