Abstract
A combined approach of subwindow factor analysis and spectral correlative chromatography was used to analyze the volatile components in Radix Flemingiae Macrophyllae and Flemingiae Latifolia Benth, one of its substitutes. After extraction by a water distillation method, the volatile components in Radix Flemingiae Macrophyllae and Flemingiae Latifolia Benth were detected by GC-MS. Then the qualitative and quantitative analysis of the volatile components in Radix Flemingiae Macrophyllae was completed with the help of subwindow factor analysis resolving two-dimensional original data into mass spectra and chromatograms. Sixty five of 82 separated constituents in the total ion chromatogram of the volatile components in Radix Flemingiae Macrophyllae were identified and quantified, accounting for about 88.79% of the total content. Then, spectral correlative chromatography was used to extract correlative constituents in Flemingiae Latifolia Benth. Fifty one correlative components were recognized in essential oil of Flemingiae Latifolia Benth. The result proves the combined approach is powerful in the analysis of complex herbal samples. The developed method can be used to compare the sameness and differences of Radix Flemingiae Macrophyllae and its substitutes and it can also be used for quality control of Radix Flemingiae Macrophyllae.
Highlights
Radix Flemingiae Macrophyllae, belongs to the leguminous plant family
The qualitative and quantitative analysis of the volatile components determined by gas chromatography–mass spectrometry (GC-MS)
Flemingiae Macrophyllae and X2 from Radix Flemingiae Latifolia Benth obtained from GC-MS, respectively
Summary
Radix Flemingiae Macrophyllae, belongs to the leguminous plant family. As a traditional Chinese medicine (TCM) listed in the Chinese Pharmacopoeia, it has been widely used to cure rheumatism, lumbar muscle strains, myasthenia of limbs, injuries from falls, swelling, sore throats, etc. [1,2,3]. Macrophyllae samples, it is still difficult to achieve a complete separation unless rigorous conditions are imposed on the chromatographic separation process In these reports, the qualitative and quantitative analysis of the volatile components determined by gas chromatography–mass spectrometry (GC-MS). Development of a simple and reliable method for the determination of the volatile constituents in Radix Flemingiae Macrophyllae and its substitutes is necessary. In this work, combined chemometrics methods, subwindow factor analysis (SFA) [14] and spectral correlative chromatography (SCC) [15], are used for analysis of the volatile constituents in Radix. After extraction by the water distillation method, the volatile components in Radix Flemingiae Macrophyllae and Radix Flemingiae Latifolia Benth. The obtained results proved the power of the developed approach for the analysis of complex samples
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