Abstract
Fuel-ethanol fermentation process includes a reutilization of the yeast biomass, in which yeast cells are exposed to stress conditions as high temperature and increasing alcohol concentration. This environment may cause a significant delay in fermentation and drop in cell viability. The Bioen program (UNESP) conducted a study to select highly producing yeast strains in a Brazilian distillery. Two of these were selected when comparable to other well established commercial yeast strains: ZFC4 (ethanol best producer) and ZFD4 (most ethanol and temperature tolerant). Based on these data the aim of this work is to study genetic features associated with cell resistance to sustained stress of selected yeast strains in synergy with standards (PE-2, CAT-1 and SA-I) used in Brazilian distillery. The yeast strain was confirmed as Saccharomyces cerevisiae using PCR. The strains were screened together for growth of 25 at 44°C on plates containing 10% and 12% (v/v) ethanol and for fermentation assays. All of these demonstrated optimal responses to the high temperature and alcohol concentration and fermentative capacity when comparable to the same yeast strain singly. To gain insight of the cellular mechanisms of resistance to these stresses, global gene expression analysis of the selected strains will be performed.
Highlights
Study of the synergistic activity between industrial yeast strains resistant to high temperature and ethanol concentrations and high fermentative capacity to produce ethanol
Fuel-ethanol fermentation process includes a reutilization of the yeast biomass, in which yeast cells are exposed to stress conditions as high temperature and increasing alcohol concentration
Two of these were selected when comparable to other well established commercial yeast strains: ZFC4 and ZFD4. Based on these data the aim of this work is to study genetic features associated with cell resistance to sustained stress of selected yeast strains in synergy with standards (PE-2, CAT-1 and SAI) used in Brazilian distillery
Summary
Fuel-ethanol fermentation process includes a reutilization of the yeast biomass, in which yeast cells are exposed to stress conditions as high temperature and increasing alcohol concentration. Study of the synergistic activity between industrial yeast strains resistant to high temperature and ethanol concentrations and high fermentative capacity to produce ethanol Strohmayer Lourencetti Natália Manuela1*, Danieli Flávia2, Valentini Roberto Sandro3, de Lucca Gattás Edwil Aparecida2, Soares Mendes Giannini Maria José1, Zanelli Cleslei Fernando3, Fusco Almeida Ana Marisa1
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
