Abstract

Currently, the development of methods for isolation, indication and identification of Pseudomonas stutzeri bacteria from environmental objects and pathological material is an urgent problem. At the same time, there are no data in the scientific literature on the sensitivity of Ps.stutzeri bacteria to various inhibitors, which are necessary for the development of a selective and differential nutrient medium for them. The article presents the results of studying the sensitivity of Ps. stutzeri and their associates to inhibitors such as sodium benzoate, SDS, nalidixic acid, potassium tellurite and sodium azide, which will be used to develop a selective and differential culture medium for Ps.stutzeri bacteria.

Highlights

  • Ps.stutzeri bacteria live in many natural habitats

  • All studied reference strains were inoculated in the GRM, and daily bacterial cultures of Ps.stutzeri, Yersinia enterocolitica, Klebsiella pneumoniae, Escherihia coli, Proteus mirabilis, Aeromonas hydrophila, Citobacterfreundii, Pseudomonas putida type, Pseudomonas putida, Pseudomonse type, as well as Pseudomonas fluorescens, Streptococcus pyogenes, Staphylococcus aureus, Listeria monocytogenes

  • On the basis of the obtained research results, it was concluded that sodium and potassium benzoate tellurite should be used as an inhibiting component of the constructed selective medium for Ps.stutzeri is impractical, as is the study of the sensitivity of the studied bacteria to their lower or higher concentrations

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Summary

Introduction

Ps.stutzeri bacteria live in many natural habitats They are isolated from sewage, ground and sea waters, treatment facilities, soil, manure, objects of the external hospital environment, and much less often from clinical materials of humans and animals [1, 2]. They are biodestructors and are capable of manifesting a pathogenicity factor with the ability to cause disease. It is necessary to develop a fast and highly efficient method for the indication and differentiation of Ps.stutzeri bacteria from other microorganisms isolated from environmental objects and pathological material. Proteas can grow in this environment, their swarming being suppressed

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