Abstract
During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. Operational conditions are considered to be one of the main parameters responsible for such changes, especially when using the same membrane and molecule. The present study demonstrates that, the same cut-off membrane (commercial data) can result in different properties of the protein after filtration, due to their different pore network. The filtration of lysozyme, regardless of the membrane, produces a decrease in the membrane hydraulic permeability (between 10 and 30%) and an increase in its selectivity in terms of observed rejection rate (30%). For the filtrated lysozyme, it appears that the HPLC retention time increases depending on the membrane used. The antibacterial activity of the filtrated samples is lower than the native protein and decreases with the increase of the applied pressure reaching 55–60% loss for 12 bar which has not been reported in the literature before. The observed results by SEC-HPLC and bacteriological tests, suggest that the conformation of the filtrated molecules are indeed modified. These results highlight the relationship between protein conformation or activity and the imposed shear stress.
Highlights
During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity
Lesnierowski et al had studied the effect of applied pressure, temperature, pH and ultrafiltration time on antibacterial activity of lysozyme[30]
A relationship between the hydrodynamic parameters, protein conformation and cell lysis activity of lysozyme was investigated in this work by ultrafiltration experiments performed at different pressures, SEC-HPLC analysis and antibacterial tests
Summary
During the processing of biomolecules by ultrafiltration, the lysozyme enzyme undergoes conformational changes, which can affect its antibacterial activity. On the other hand, are a greater alternative Their advantages include low energy consumption, high selectivity, continuous possibility of separation, mild conditions of operation and the possibility to avoid any additional chemicals (additives). Ultrafiltration (UF) is a common choice for membrane process as it can be used concomitantly for protein concentration, buffer exchange, desalting, protein purification and viruses and bacteria c learance[9]. It is a pressure driven process for molecules with a molecular weight from 50 to 500 kg mol−1 and diameter of 1–100 nm[10]. Besides that, depending on the feeding solution, it is possible to choose different membrane materials varying from organic
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