Abstract
We previously reported that when the high-functioning human hepatoma cell line, FLC-5, immortalized sinusoidal endothelial cell line, M1, and immortalized hepatic stellate cell line, A7, were cultured in the 3-dimensional filled type bioreactor, tissue reorganization resembling that seen in the live liver occurred, with the appearance of pores in the sinusoidal endothelial cells (SECs) [1]. The process and mechanism of formation of these pores remain unclarified. The presence of actin at the margin of these pores has been demonstrated by electron microscopic study [2]. Swinholide-A, which is actin inhibitor synthesized from Okinawa sponge, increase the number of pores on primary culture on SECs derived from the rat [3]. In present study, we examine whether or not the pores on SECs under three-dimensional perfusion co-culture treatment with Swinholide-A behave like those in primary culture cells.
Highlights
We previously reported that when the high-functioning human hepatoma cell line, FLC-5, immortalized sinusoidal endothelial cell line, M1, and immortalized hepatic stellate cell line, A7, were cultured in the 3-dimensional filled type bioreactor, tissue reorganization resembling that seen in the live liver occurred, with the appearance of pores in the sinusoidal endothelial cells (SECs) [1]
We examine whether or not the pores on SECs under three-dimensional perfusion co-culture treatment with Swinholide-A behave like those in primary culture cells
After perfusion for 1 hour with medium supplemented with 100 nM Swinholide-A, or for 2 hours with the medium supplemented with 200 nM Swinholide-A, the cellulose beads along with the adherent cells were withdrawn from bioreactor
Summary
We previously reported that when the high-functioning human hepatoma cell line, FLC-5, immortalized sinusoidal endothelial cell line, M1, and immortalized hepatic stellate cell line, A7, were cultured in the 3-dimensional filled type bioreactor, tissue reorganization resembling that seen in the live liver occurred, with the appearance of pores in the sinusoidal endothelial cells (SECs) [1]. The presence of actin at the margin of these pores has been demonstrated by electron microscopic study [2]. Swinholide-A, which is actin inhibitor synthesized from Okinawa sponge, increase the number of pores on primary culture on SECs derived from the rat [3]. We examine whether or not the pores on SECs under three-dimensional perfusion co-culture treatment with Swinholide-A behave like those in primary culture cells
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