Study of the Protein Patterns under the Effect of Spraying with Moringa Plant Extract and Potassium Silicate for Banana Obtained from Tissue Culture Musa spp

Abstract

The field experiment was conducted in one of the greenhouses affiliated with the College of Agriculture and Marshes - University of Thi-Qar during the growing season 2022-2023 on banana seedlings, the Grand Nine variety, to know the effect of Moringa extract and potassium silicate on the protein patterns of seedlings. The study included two factors. The first factor was spraying with an extract. Moringa leaves in four concentrations (0, 5, 10, 15) g L-1, the second factor: spraying with potassium silicate in four concentrations (0, 1, 2, 3) ml L-1, the first spraying appointment was at an interval of ten days between one spray and another, by 6 sprays, the experiment was applied according to the complete random design (C.R.D) with three replications. The results for the protein patterns were as follows. All treatments participated in the presence of two protein bundles that shared the same molecular weight as the first protein bundle, which amounted to 143.724 kDa. It also shared the same molecular weight as the second protein bundle, which recorded a molecular weight of 93.595 kDa. It gave four protein bundles in the comparison treatment and five protein bundles. Only for all treatments of Moringa extract and all potassium silicate treatments with molecular weights ranging between 7.619-15.465 kDa, and when the interaction between Moringa extract at a concentration of 5g L-1 with all concentrations of potassium silicate caused an increase in the number of protein bundles to six bundles whose molecular weights ranged between 7.857-8.862 kDa, as for the interaction between Moringa extract at two concentrations of 10 and 15 g L-1 with all potassium silicate concentrations (except for concentration 3 ml L-1), it caused an increase in the number of protein bundles to seven, with molecular weights ranging between 7.698-8.254 kDa. While the interaction between Moringa extract 15 g L-1 and potassium silicate at a concentration of 3 ml L-1 caused an increase in the number of protein bundles to eight bundles with different molecular weights, the molecular weight of the eighth bundle was 7.619 kDa.