Abstract
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children. Materials and research methods. A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No. 6 in Novosibirsk in the first 2 days from the onset of the disease. Two groups were formed — group 1 — patients with diarrheal syndrome and signs of respiratory system damage. Group 2 — children with isolated lesions of the respiratory tract. In all children, the genetic material of viruses was determined by polymerase chain reaction (PCR) in the nose and throat washes. Also, in all children, the determination of serum levels of IL-8, IL-17, IFN-ã by ELISA was performed. Results and discussion. When studying the levels of cytokines (IFN-ã, IL-8, IL-17) in patients with signs of gastrointestinal tract lesions in comparison with isolated respiratory manifestations, no statistically significant differences were found. However, when analyzing the levels of the studied parameters in patients with diarrheal syndrome in different age groups, significant differences in the levels of IFN-ã and IL-8 were revealed.
Highlights
Study of the production of some cytokines in viral infections occurring with diarrheal syndrome in children
The aim of the study was to study the characteristics of the content of certain cytokines in serum as indicators of the immune response in viral infections accompanied by diarrhea syndrome in children
A survey was conducted of 92 children aged 3 months to 15 years with acute respiratory viral infections hospitalized in hospital No 6 in Novosibirsk in the first 2 days from the onset of the disease
Summary
Для решения поставленной задачи проведено обследование 92 детей с острыми респираторными вирусными инфекциями, госпитализированных в стационар ДКБ No6 города Новосибирска в первые 2—3 суток от начала заболевания. Критерием включения для I группы (n = 71) являлось наличие признаков острой респираторной инфекции, а также наличие признаков поражения желудочно-кишечного тракта (диарейный синдром). Критерием включения для II группы (n = 21) было изолированное поражение. Уровень содержания цитокинов в группах без учета возраста (М ± m) Table 1. У всех детей было проведено определение содержания в сыворотке крови IL-8, IL-17, IFN-γ. Определение концентраций цитокинов — IL-8, IL-17, IFN-γ в сыворотке крови выполняли с использованием коммерческих тест-систем для иммуноферментного анализа производства ООО «Цитокин» (Россия) по инструкции производителя. В группе детей с ОРВИ у всех законных представителей пациентов (родители) было получено добровольное информированное согласие на забор биологических жидкостей, а также использование данных исследования в научных целях. Абсолютные значения исследованных количественных показателей представлены в виде средних величин и их стандартной ошибки (М ± m). Минимальную вероятность справедливости нулевой гипотезы принимали при 5% уровне значимости (р < 0,05)
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