Study of the Physiochemical and Functional Properties of Fenugreek Protein Isolate

Abstract

The aim of the research was to isolate Fenugreek Protein from local defatted seed powder and to investigate its physical and functional properties. The results showed that protein yield was 2.3% on dry weight basis, physical and functional properties results where the Isoelectric point of fenugreek protein is 4.5. The highest solubility was recorded at pH 2,10 56.60-%,58.0% respectively. the highest emulsification ability was at pH10=36% and at pH 3=35%. fenugreek protein isolate has a water holding capacity 2.46 ml / g. The oil binding capacity depends on the presence of hydrophobic amino acids. The oil binding capacity was 6.26 ml / g. Fenugreek protein isolate with 90.14% protein content was extracted from fenugreek seeds, Traces of fat (1.285), fiber (0.10%), carbohydrate (0.75%), ash (1.53%). Also, FTIR and spectroscopy was used to characterize the Fenugreek protein isolate powder, (Protein was isolated from Iraq fenugreek seeds and converted to powders using freeze drying)