Abstract
Background and study aim : ELISA can determine specific antibody classes against brucella, It is a sensitive, simple and rapid test, thus help to study the pattern of the brucellosis. The aim of this article is to study the pattern of the brucellosis in Menoufyia governorate. . Patients and Methods: Sera from 150 individuals from confirmed brucellosis cases and 25 healthy individuals were tested for presence of IgG and IgM antibodies by ELISA assay. Culture positivity for brucellosis was used as the reference standard for diagnosis. Results: Serum IgG and IgM for brucellosis by ELISA test had increased values in confirmed brucella cases, ELISA IgM was highly specific (100%) in all groups and sensitive (96%) in acute brucellosis, (100%) in subacute brucellosis and (64%) in chronic brucellosis. While, ELISA IgG specificity in all groups was (80%) and the sensitivity in acute brucellosis was (88%), in subacute and chronic brucellosis was (100%). Conclusion: ELISA IgG and IgM test for brucella is a simple and reliable test in the diagnosis of pattern of the brucellosis.
Highlights
ELISA IgM test was positive in all patients with subacute brucellosis (100%), most patients with acute brucellosis (95.5%), most patients with chronic brucellosis (64%), as shown in table (7) while, ELISA IgG was specific in all groups is (80%) and the sensitivity in acute brucellosis was (87.6%), in subacute and chronic brucellosis was (100%) as shown in table (8)
In the present study we found that, human brucellosis affects all age groups as shown in table (1) this agree with Cetinkaya1 et al [7] who found that human brucellosis affects all age groups
ELISA IgM test was positive in all patients with subacute brucellosis (100%), most patients with acute brucellosis (96%), most patients with chronic brucellosis (64%) and negative in all persons in the control group as shown in table (7) and ELISA IgG test was positive in most patients with brucellosis whatever the duration of disease and negative in most persons in the control group as shown in table (8) This result agree with Maha et al [18] who found that, the examined cases using ELISA of clinically suspected brucellosis yielded positive result with ELISA, (80%) were positive for brucella IgM while, (64.6%) were positive for brucella IgG
Summary
Brucellosis is a worldwide zoonosis caused by the facultative intracellular members of the bacterial genus Brucella of which at least six species are recognized; these are highly genetically homogenous and the disease is of major socio-economic importance [1].Brucellosis is a significant health problem in Egypt and a confirmed cause of 3% of acute febrile illnesses [2].The estimated annual incidence of brucellosis in Egypt per 100000 population was 64 and 70 in 2002 and 2003, respectively [3].Diagnosis of the disease is challenging and is frequently delayed or missed because the clinical picture may mimic other infectious and non-infectious conditions, It is often missed because of its non-specific symptoms unless the clinician is aware of the organism and maintains a high degree of suspicion so, diagnosis can be established by laboratory methods such as, serology and blood cultures [4].In absence of bacteriologic confirmation, a presumptive diagnosis can be made on the basis of high or rising titers of specific antibodies [5].accurate diagnosis of brucellosis is vital for early institution of proper therapy as untreated cases may progress to chronic stage and focal complications [6]. Brucellosis is a worldwide zoonosis caused by the facultative intracellular members of the bacterial genus Brucella of which at least six species are recognized; these are highly genetically homogenous and the disease is of major socio-economic importance [1]. Brucellosis is a significant health problem in Egypt and a confirmed cause of 3% of acute febrile illnesses [2]. In absence of bacteriologic confirmation, a presumptive diagnosis can be made on the basis of high or rising titers of specific antibodies [5]. ELISA can determine specific antibody classes against brucella, It is a sensitive, simple and rapid test, help to study the pattern of the brucellosis. The aim of this article is to study the pattern of the brucellosis in Menoufyia governorate
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