STUDY OF THE MASS-SPECTORS’ FEATURES OF SPORES AND VEGETATIVE FORMS OF BACILLUS ANTHRACIS BY THE METHOD OF TIME OF FLIGHT MASS-SPECTROMETRY

Abstract

Aim. Investigation of the features of the protein profiles of the spore and vegetative form of the anthrax microbe by the MALDI-TOF MS method using the resources of the Mass-Up program and the package for the statistical software with open source code R. Materials and methods. Spores and vegetative forms of 32 strains of B. anthracis from the collection of microorganisms of the Stavropol Research Institute for Plague Control, including 8 strains isolated from an outbreak of anthrax in Yamal in 2016. Protein profiling was carried out on the Microflex MALDI-TOF mass spectrometer «Bruker Daltonics». Results. The alternative bioinformational-statistical approach used to analyze the MALDI-TOF mass spectra of the causative agent of anthrax made it possible to differentiate the spores and vegetative forms of the microbe based on the identification of the corresponding groups of biomarkers. Conclusion. A comparison of vegetative and spore cells of typical and atypical strains of anthrax causative agent on the basis of MALDI-TOF MS data was made. It has been experimentally confirmed that the protein profiles of cultures of Bacillus anthracis of the spore and vegetative form differ significantly, and this difference can be used to search for potential markers of each of the forms.