Abstract
A new assay of salmon sperm DNA at nanogram level was established based on enhanced resonance light scattering (RLS) signals of DNA with myricitrin and cetylpyridinium bromide dihydrate (CPB). The RLS spectral characteristics of DNA with myricitrin–CPB and the optimum conditions for determination of DNA samples have been studied. At pH 7.4, myricitrin–CPB could enhance the intensity of RLS signal of DNA at 468nm. The enhanced RLS intensity was directly proportional to DNA concentration in the range of 0.076–4.2μgmL−1 with a good linear relationship (r=0.9944). The detection limit was 4.1ngmL−1. The synthetic samples were analyzed with satisfactory results that the recovery was 100.9–102.6% and RSD was 1.4–2.1%, which proved that the new method was reliable and applicable.
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