Study of the interaction between indole-based compounds and biologically relevant G-quadruplexes

Abstract

G-quadruplexes (G4s) are high-order secondary structures that modulate several key cell processes such as telomere function, gene expression and DNA replication. In the past years G4s have emerged as promising targets for drug development due to the discovery of small molecules which bind and stabilize these structures. In this work, we report the synthesis of indole-based compounds and the study of their interaction with the biological relevant G4s c-MYC and human telomeric repeat 22AG using several biophysical techniques. The ligands are G4 specific and they can discriminate different G4 structures namely parallel and hybrid-1 topologies. The NMR study of interaction between the most promising indole-derivative (compound 3) and c-MYC quadruplex suggests that the ligand binds on the external tetrads with additional actions in the loops/grooves in a 2:1 ratio. The molecular docking calculations of compound 3 to c-MYC quadruplex corroborates with the 1H and NOESY NMR studies.Overall, the results suggest that indole-based ligands are promising candidates for future lead optimizations in drug development.