Study of the interaction between a persistent infectious pancreatic necrosis virus (IPNV) infection and experimental infectious salmon anaemia (ISA) in Atlantic salmon, Salmo salar L.

Abstract

Abstract. An infectious pancreatic necrosis virus (IPNV) carrier stock of Atlantic salmon parr (100 g) was divided between two tanks and inoculated experimentally with tissue homogenate containing the aetiologic agent of infectious salmon anaemia (ISA) and non‐ISA tissue homogenate (control), respectively. Plasma and kidney samples from ISA‐infected and control fish were taken twice weekly for 25 days. In the kidney samples, IPNV was quantified by a plaque assay. In plasma, anti‐IPNV antibodies were measured using an indirect ELISA. The ISA‐infection did not seem to activate the IPNV‐infection. Neither the proportion of fish with IPNV or anti‐IPNV antibodies, nor the IPNV titre or level of anti‐IPNV antibodies showed any specific trend during the study. Independently of ISA, IPNV was detected in 54 out of 132 fish (41%), while 71 out of 195 fish (36%) had plasma antibodies against IPNV. No association was found between detection of IPNV, and presence or level of anti‐IPNV antibodies in individual fish.