Abstract
An experimental study of biodegradation of Shenmu coal was carried out by using Ochrobactrum cytisi, Novospingobium naphthalenivorans, Alcaligenes faecalis and Pseudomonas fluorescens. The micro-nano pore structure of coal samples before and after biodegradation was studied by low-temperature N₂ adsorption. For biodegraded coal, the results showed that micropores and mesopores are primarily open pores with good connectivity, including parallel plate pores and cylinder pores with two open ends; the specific surface area of biodegraded coal decreased from 2.2174 m²/g to 1.6255˜2.0537 m²/g, and the pore size of the coal biodegraded by the four bacteria decreased following biodegradation from 250 nm to 170˜200 nm, which may be due to collapse of the coal structure due to the bacterial degradation. Coal biodegradation by the dominant bacterium P. fluorescens led to a diminished mesopore size and an increased number of smaller mesopores, with the smaller mesopores gradually taking on dominant roles.
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