STUDY OF THE INFLUENCE OF BACTERIAL LYSATE ON INDICATORS OF LYSOSOMAL PHOSPHATASE ACTIVITY IN EXPERIMENTAL PERIODONTITIS IN RATS

Abstract

Introduction. One of the stages of the pathophysiological study of the parameters of mineral metabolism of alkaline (ALP) and acidic (AP) phosphatases – enzymes of bone tissue of osteoblast and osteoblast membranes in experimental periodontitis against the background of the use of bacterial lysate Respibron® was carried out. Materials and methods. Experimental periodontitis in rats was in the modification of O.I. Sukmansky. There were rats with experimental periodontitis and rats treated by Respibron (10 mg/kg 1 time per day) or Imudon for 30 days. Determination of alkaline (ALP) and acidic (AP) phosphatases activity (pH 4.8 and 10.5) was performed according to the methodological recommendations of AP Levitsky et al. Discussion of results. It was found that by the end of the 90-day period of the experiment, a statistically stable course of chronic damage to the periodontium was observed in terms of ALP and AP, which exceeded the intact ones by 1.93 and 1.4 times, respectively, which reflects the hypoergic state of chronic periodontal inflammation. A powerful corrective effect of the drugs Respibron® and Imudon® on markers of osteoblast and osteoblast activity in experimental periodontitis has been proven. Established a stimulating effect on osteoregeneration Respibron® in the hypoergic course of the inflammatory process. The therapeutic effect of Respibron® is more expressive and powerful compared to Imudon®. Conclusions.The results obtained make it possible to continue the study of the course of experimental periodontitis in order to establish the mechanisms of development, protection, and restoration of tissues under the conditions of the pharmacological correction Respibron®.