Abstract
ABSTRACT The effect of several factors on in vitro rooting of the Kazanlak oil-bearing rose has been studied. Using the Murashige Skoog nutrient medium, the concentrations of naphthalene acetic acid (0.05 and 0.1 mg/L), the presence of phloroglucinol and variation in the quantity of mineral salts used were evaluated. Also, the conditions of microplant cultivation such as temperature, light and photoperiod, as well as the changes during growing in a two-phase-nutrient medium (solid, including the tested variants, plus liquid) were studied. Optimum results were obtained using a nutrient medium with 25% of the recommended mineral salts normally encountered in the medium plus 0.1 mg/L naphthalene acetic acid and 126 mg/L phloroglycinol in the presence of light. Analogous results were obtained for both the single and two-phase-nutrient media.
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