Study of the immunomodulatory role of heteroglucan on a 3D tumor spheroid and macrophage co-culture milieu

Abstract

Event Abstract Back to Event Study of the immunomodulatory role of heteroglucan on a 3D tumor spheroid and macrophage co-culture milieu K. Sanjana P. Devi1 and Tapas K. Maiti1* 1 Indian Institute of Technology Kharagpur, Department of Biotechnology, India Over the past several years, glucans have garnered much attention due to their immunomodulatory properties. Till date most studies of glucans has been done in vivo or in two dimensional cultures. A 3D platform may thus serve as a tool to optimize drug effects in tissue environment as well as reduce animal testing and economical wastage. The present study was focussed on establishing a three dimensional tumor spheroid model and its co-culture with glucan (isolated from Pleurotus ostreatus mycelia) stimulated macrophages to mimic the complexity retained in tumor tissues and the immunological phenomena of stimulated phagocytes. Spheroids of HT-29 were incubated with glucan activated human THP-1 macrophages at an effector:target cell ratio of 1:1 and the invasive property of the phagocytes were examined. Interestingly, an increase in the migration and penetration of activated macrophages to the tumor spheroids was observed by confocal microscopy using a two dye staining method. Concomitantly, immunohistological studies further corroborated these facts whereby activated macrophages were found to deeply penetrate the spheroidal layer. Tumor cell death was also found to be increased in the peripheral zones of spheroids incubated with glucan treated macrophages. The degree of distortion of the spheroidal morphology attained higher growth with an increase in the co-culture incubation period. Untreated macrophages apparently exhibited lower invasiveness, death and distortion of the tumor spheroids. Spatiotemporal and micro environmental influences in the behaviour of cells monitored in such 3D system are therefore prospects to be accounted for deeper understanding of the complex immune network. Keywords: glucan, spheroid, macrophage, co-culture, immunological Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Translational immunology and immune intervention Citation: Devi KP and Maiti TK (2013). Study of the immunomodulatory role of heteroglucan on a 3D tumor spheroid and macrophage co-culture milieu. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00257 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Prof. Tapas K Maiti, Indian Institute of Technology Kharagpur, Department of Biotechnology, Kharagpur, India, tkmaiti@hijli.iitkgp.ernet.in Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers K. Sanjana P Devi Tapas K Maiti Google K. Sanjana P Devi Tapas K Maiti Google Scholar K. Sanjana P Devi Tapas K Maiti PubMed K. Sanjana P Devi Tapas K Maiti Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.