Study of the genetic stability of plant potatoes grown outside the living body after years of repeated cultivation using the technology of randomized reaction of DNA

Kholod Hassen,Jaladet Salah

doi:10.33899/edusj.2007.162842

Abstract

Random Amplified Polymorphic DNA markers were used to evaluate the· genetic stability of micro propagated plants of potato (Solanum tuberosum) var.Desiree .To achieve this genomic DNA wasextracted from the samples to comparison between the DNA of plantlet micro propagated through meristem culture and the DNA of the plantlets (7 samples) after sub culturing 16 times, beside the comparison within thesamples. Fifteen arbitrary primers were used to amplify DNA from the samples, to assess the genetic stability .All RAPD profiles from micro propagated plants were mono morphic and similar to the DNA of the origin sample.no variation was detected within the micro propagated plants, this prove that potato plants used in this study were resistant to somaclanal variation and utilization of RAPD markers for the assessment of genetic stability.

Highlights

GTGAGGCGTC TGTCTGGGTG TGTCATCCCC GACTGGACAC ACTGAACGCC TCGCCGCAAA ACATCGCCCA AACGGCGACA GGTCTGGTTG CAGACAAGCC (SJ4ll ~' OPC.02 OPC.10 OPC.12 OPL.03 OPN.05 OPN.ll OPP.08 OPT.08 OJ>X.09 OPX.15 bp ))ijij
Desiree .To achieve this genomic DNA was extracted from the samples to comparison between the DNA
of plantlet micro propagated through meristem culture

Summary

Introduction

GTGAGGCGTC TGTCTGGGTG TGTCATCCCC GACTGGACAC ACTGAACGCC TCGCCGCAAA ACATCGCCCA AACGGCGACA GGTCTGGTTG CAGACAAGCC (SJ4ll ~' OPC.02 OPC.10 OPC.12 OPL.03 OPN.05 OPN.ll OPP.08 OPT.08 OJ>X.09 OPX.15 bp ))ijij. ~ w~l j\ .DNA Jl dJ...J....J ~1~1 jc.ls:JI ~ o~IDNAJI .u...uL..J ~1~1 (.·.).)..) ~ . .;;II ~..- .r·-·'I~~ _)_·) l)·A ~\...:JI t.blh.J. I wl.:u..u l.-)A DNAJI ~~~ ~ '- ~~. ~ ~jSJ w~l ~ ~I.JJ wly,~ .J~ r-jc .)c J~ I~J ~'il DNAJI .J...>:WI _#J ~J.J.JA oj~ ufo ~IJ (Somaclonal variation) ~I.J)I wl_;.;loll t=:-"Y-J ,wlY-loll ~.by.o.

Results

Conclusion