Study of the gene pool of the winter rye Secale cereale L. of the Republic of Belarus using microsatellite markers

V S Mandrusova,I A Gordej,O M Lyusikov,V E Shimko,I S Gordej

doi:10.29235/1029-8940-2021-66-2-215-222

Abstract

In this work, the genetic diversity of the modern gene pool of the winter rye (S. cereal L.) of the Republic of Belarus from 20 actual breeding samples was investigated using 15 microsatellite (SSR) markers to develop divergent crossing combinations in breeding for heterosis. It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. The most effective microsatellite markers (SCM28, SCM43, SCM101 and SCM102) were identified and can be successfully used to study the genetic diversity of rye. It has been established that the modern gene pool of the winter rye of the Republic of Belarus is generally characterized by fairly wide genetic diversity (interpopulation variability) – all collection samples are characterized by a unique allelic composition of the studied microsatellite loci. Based on investigation results, a hierarchical clustering dendrogram was constructed, which made it possible to determine the most genetically divergent combinations of crosses. The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis.

Highlights

It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72
The information obtained can be used for the development of an effective scheme allowing to develop new varieties and hybrids in the practical breeding of rye for heterosis
Сегодня рожь выращивается на площади всего около 300 тыс. га и дает ежегодно 0,7‒0,8 млн т зерна, хотя в 1990-х годах посевные площади под этой культурой в нашей стране доходили до 900 тыс. га [4]

Summary

Introduction

It was shown that the formed set of SSR markers is highly effective – the informational content index (PIC) varied from 0.50 to 0.83 and averaged 0.72. За последние десятилетия накоплен большой теоретический и практический опыт использования ДНК-маркеров в генетике и селекции растений как вспомогательный метод скрининга генофонда исходного материала при создании коммерческих сортов и селекционных линий различных сельскохозяйственных культур [6]. Цель исследований ‒ изучение генетического разнообразия современного генофонда озимой ржи Secale cereale L.

Results

Conclusion