Year-end Sale % OFF 
Abstract
The aim of the study was the characterization of the electrophoretic behavior of cephalosporins from different generation having different structural characteristics in order to develop a rapid, simple and efficient capillary electrophoretic method for their identification and simultaneous separation from complex mixtures. Ten cephalosporin derivatives (cefaclor, cefadroxil, cefalexin, cefazolin, cefoxitin, cefuroxime, cefoperazone, cefotaxime, ceftazidime, ceftriaxone) were analyzed by capillary zone electrophoresis using different background electrolyte solutions at different pH values. Electrophoretic mobilities of the analytes were calculated, the influence of the electrophoretic parameteres on the separation was established and the analytical conditions were optimized. Taking into consideration their structural and chemical properties cephalosporins can be detected over a pH range between 6 and 10. The best results were obtained using a buffer solution containing 25 mM disodium hydrogenophosphate - 25 mM sodium dihydrogenophosphate, at a pH - 7.00, + 25 kV voltage at a temperature of 25 °C, UV detection at 210 nm. Using the optimized analytical conditions we achieved the simultaneous baseline separation for seven cephalosporins in less then 10 minutes. Using the described optimized electrophoretic procedures, capillary electrophoresis can be used for the identification and determination of cephalosporins in formulated pharmaceutical products and for their separation from complex mixtures.
Highlights
Nowadays cephalosporins are one of the most important and probably the most frequently used antibiotics in the world, both in terms of the number of compounds currently on the market as well as regarding their use in the treatment of infectious diseases
In this study 10 cephalosporins from different generations having different structural characteristics were analyzed by capillary zone electrophoresis (CZE): 1st generation cephalosporin derivatives, 2nd generation cephalosporin derivatives, 3rd generation cephalosporin derivatives
Preliminary study In order to obtain the optimum capillary electrophoretic conditions for the determination of cephalosporins, the electrophoretic mobilities of the analytes were determined at different pH values using different background electrolytes: 25 mM phosphoric acid, 25 mM sodium didydrogenophosphate, 25 mM disodium hydrogenophosphate – sodium didydrogenophosphate (1:1) and 25 mM sodium tetraborate; the pH of the buffers was adjusted to the desired pH by adding a 0.1M sodium hydroxide solution
Summary
Nowadays cephalosporins are one of the most important and probably the most frequently used antibiotics in the world, both in terms of the number of compounds currently on the market as well as regarding their use in the treatment of infectious diseases. High performance liquid chromatography (HPLC) remains by far the most frequently used method in the analysis of cephalosporins, capillary electrophoresis (CE) is being increasingly employed due to its favorable characteristics (high separation efficiency, large flexibility, rapid method development and low consumption of samples and reagents).[6,7,8] Separating simultaneously different types of cephalosporins from different generations remains a CE has been successfully applied for the analysis of cephalosporins from different pharmaceutical forms,[15] environmental samples,[14] plasma,[9] blood serum,[20] urine and bile,[10] bronchial secretion,[13] and in stability studies[12] and for the determination of dissociation constants.[4,5] While MEKC proved to be especially useful for the determination of cephalosporins from biological samples reducing the disadvantageous matrix effects caused by organic materials, CZE proved to be a powerful analytical tool for determinations from pharmaceutical products.[10,13,15,19] Structurally cephalosporins are a rather heterogeneous group;[2] a large number of electrophoretic procedures using different analytical parameters can be used for their separation. This paper presents a comprehensive study of the electrophoretic behavior of cephalosporins from different generations and the development of a generic simulataneous separation method of several cephalosporins from complex mixtures
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
