Study of the effects of butyric acid and propanediol of the intestine in ICR mice

Abstract

The effect of gut microbiota correctors on the motility of the gastrointestinal tract of ICR mice was studied. Two feed additives were used - compositions based on glycerol and 1.3 propanediol and Salkoli Mono BP Dry. Three groups of 10-12 animals each were formed according to the principle of analogues: two experimental and one control. Experimental feed mixtures were made by saturating 200 g of feed with sunflower oil, 1.3 propanediol, glycerol (experimental group 1); the feed mixture of experimental group 2 was additionally mixed with the feed additive Salkoli Mono BP Dry. To estimate the rate of fecal excretion, 100 µl of fluorescent tags (fluorescent ink green and red) were given to mice of all groups, previously mixed with water 1: 1. The timing of the appearance of the first signs of fluorescence in feces in animals of the control and experimental groups was estimated. Intensive fluorescence of the green label in fluorimetry samples was observed. The greatest increase in fluorescence was observed at the 4th hour of the experiment in the control group, while in the 2nd experimental group the increase lasted until the 5th hour. In the 1st experimental group, elimination of the main amount of green dye did not begin even by the 5th hour of the experiment. The method using fluorescent tags revealed a delay in mixing of feed masses in mice fed glycerol and 1,3 propanediol, which was accompanied by a statistically significant increase in Escherichia coli concentration - by 3 times (p < 0.05). The use of Salkoli Mono BP Dry had no statistically significant effect on E. coli concentrations and gastrointestinal motility.