Study of the Effect of Nutrient Environment on the Biological Characteristics of Fusarium solani Clinical Corneal Isolates

Dalan Jing,Wei Wang,Yingyu Li,Ziyuan Liu,Chen Huang,Pei Zhang,Alessandro Meduri

doi:10.1155/2021/6666303

Abstract

Purpose The biological characteristics of Fusarium solani clinical corneal isolates growing in different nutritional conditions in vitro were studied in order to find out the key point of pathogenicity. Methods Five kinds of media with different glucose and nitrogen concentrations were prepared as the liquid and solid forms. The clinical isolates were as follows: 2 Fusarium solani strains. The clinical corneal isolates and the standard strains were inoculated in the solid and liquid media. They were all incubated at 296 for 96 h and observed at defined time points. The optical density was recorded to generate the growth curves in liquid media. Morphologic changes of colonies in the solid media were determined under the light microscope. Results The clinical isolates of Fusarium solani showed stronger reproductive capacity in the abominable nutritional condition. Besides, when the glucose concentration in the medium was consistent with the glucose concentration of aqueous in diabetic patients, the clinical isolates would show the biological features of quicker growth rate and stronger reproductive capacity. Conclusions Nitrogen source is essential for fungus reproduction. The clinical isolates showed stronger environmental adaptability under different nutritional conditions and more sensitive to environmental changes.

Highlights

Ocular fungal infections have been recognized as important causes of blindness and morbidity
Patients with fungal keratitis are immunocompetent compared with other patients who have pulmonary or systemic fungal infection. us, keratitis patients mount a variety of immune reactions to fungi [6, 7]
For S2, clear difference in growth was observed compared to CGMCC3.5840. e culture grossly showed smaller than the standard strains

Summary

Introduction

Ocular fungal infections have been recognized as important causes of blindness and morbidity. Keratitis is by far the most frequent fungal infection of the eye [4]. Patients with fungal keratitis are immunocompetent compared with other patients who have pulmonary or systemic fungal infection. Us, keratitis patients mount a variety of immune reactions to fungi [6, 7]. E clinical outcomes of fungal keratitis are worse than bacterial keratitis due to delay diagnosis, abuse of antibiotics or steroids, fungal virulence, and few antifungal drugs [8]. Fungal keratitis can lead to serious consequences; the majority of patients with moderate or worse visual impairment and about 25% patients need corneal transplantation [9]. Fungal keratitis is a major blinding eye disease in Asia [10]. Clinical corneal isolates might differ from standard strains in many ways

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Discussion

Conclusion