Study of the effect of gallic acid and cold plasma on the levels of inflammatory factors and antioxidants in the serum sample of subjects with type 2 diabetes mellitus

Abstract

Background: Uncontrolled type 2 diabetes mellitus can have devastating consequences. The role of functional foods in controlling and even preventing diabetes mellitus is prominent, and adjunct therapies can be helpful in controlling some of the consequences of diabetes.Objective: The aim of this study was to investigate whether gallic acid, as a functional food, as well as cold atmospheric plasma, as an adjunct therapy, have an effect on the levels of some antioxidant enzymes, inflammatory factors, and the levels of oxidizing agent and blood glucose.Methods: In this study, 30 healthy individuals, as the control group, and 30 individuals with type 2 diabetes mellitus were selected. Samples of people with diabetes were examined before and after treatment with gallic acid and cold atmospheric plasma (cold argon plasma jet for 10 minutes). Levels of interleukin 2 and 13 and NF-κB, as inflammatory factors, glutathione reductase, paraoxonase, and lipoprotein lipase, as antioxidants, hydrogen peroxide and blood glucose were assessed in untreated and treated diabetic groups and control group according to kit instructions.Results: Comparison of the results of the levels of inflammatory factors, antioxidants, blood glucose, and hydrogen peroxide showed a significant difference (P value < 0.05) between the diabetic and control groups. Treatment of diabetic specimens with plasma and gallic acid showed a significant increase (P value < 0.05) in glutathione reductase, paraoxonase, and NF-κB levels compared to the untreated diabetic group.Conclusions: The results showed that concomitant use of gallic acid, as well as plasma therapy, could be effective on NF-κB, glutathione reductase, and paraoxonase levels.Keywords: Diabetes mellitus, Cold plasma, Inflammatory factors, Gallic acid