Study of the effect of crude extracts and oil extract from the seeds of the (Carum carvi L.)plant on the cancerous cellular lines L20B and SR

Abstract

Caraway (Carum carvi L.) is an important medicinal plant that contains a range of bioactive compounds. The extraction process was carried out using four separate solvents: 70% methanol, 70% ethanol, 70% hexane, and water by continuous extraction using the Soxhlet device, has been chemical detection of effective ingredients in crude extracts. The volatile oils were also extracted by the aqueous distillation method using the Clevenger device and the chemical compounds found there were detected using GC-Mass technique. The study has been effect of active Components for the crude extracts and oil extracted for the seeds of the Caraway plant on the growth of the L20B,SR cancerous cellular lines out of the living body (in vitro) using two concentrations (12.5, 25)µg/ml within a 72-hour exposure period. The results showed a incorporeally difference and a probability level of P < 0.05 for some crud extracts. Where the highest rate of inhibition on the cancerous cell line L20B at the concentration of 12.5 μg/ml for ethanol extract of 29.7%, as for the SR cancerous cell line, it was the highest inhibition for extract Hot water at low concentration of 73.28%. The extracted oil also had an effect on the growth of the cancer cell lines under study. The objective of this study is to test the toxicity of crud extracts and oil to the seeds of the Caraway plant on the L20B and SR, and to know the amount of oil in the seeds of the Caraway plant