Study of the dynamics of relative expression of the EGR1 gene in rainbow trout in different tissues

Abstract

The purpose of this study was to study the dynamics of the comparative level of expression of the EGR1 gene in the tissues of rainbow trout at different ages. The EGR1 gene encodes a transcription factor that influences the transcription of a large number of genes from both the 5-bar and 3-bar ends of genes. A random sample of fish aged 6, 12 and 18 months was taken for the study. To assess the expression level of the EGR1 gene, located on 14 chromosomes, a housekeeping gene was taken: G6PD. A comparative analysis of the expression level of the EGR1 gene in rectal tissues revealed a significant change in different age periods of rainbow trout. Expression in the tissues of the rectum at the age of 6 months was maximal, and at the age of 12 months it was minimal, but no significant differences were found (p = 0.2648). As a result of the analysis of changes in the level of relative expression of the EGR1 gene in the heart tissues of rainbow trout at different ages, it was found that in the group of fish aged 6 months, the expression level was maximum at the stage of 12 and 18 months (р < 0.01). As a result of the study, a correlation between the size and weight parameters of rainbow trout and the level of gene expression in different eras and in different countries was established. Expression dynamics at different ages in rainbow trout were also determined.