Abstract
Transcriptomes, genomes, and proteomes have played important roles in the search for drug targets. To determine the molluscicidal mechanism of pedunsaponin A against Pomacea canaliculata, RNA-seq technology was adopted to analyze the differentially expressed genes (DEGs) in the P. canaliculata transcriptome after treatment with pedunsaponin A. As a result, 533 DEGs were identified, among which 255 genes were significantly upregulated and 278 genes were significantly downregulated. According to the analysis of Gene Ontology (GO) functions, we found that the DEGs were significantly enriched in the viral life cycle, UDP-glucose 4-epimerase activity, guanylate cyclase activity, the cyclic guanosine monophosphate (cGMP) biosynthetic process, and the cGMP metabolic process. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway results showed that the DEGs were mainly involved in the hedgehog signaling pathway, phagosome, cytosolic DNA-sensing pathway, retinoic acid-inducible gene I like (RIG-I-like) receptor signaling pathway, bacterial secretion system, and nuclear factor-kappa B (NF-kappa B) signaling pathway. The above results indicated that pedunsaponin A causes a metabolic disorder, anomalous opening of membrane ion channels, and an imbalance in osmotic pressure between the interior and exterior of cells, eventually resulting in the death of cells involved in immune defense and influencing the immune response of P. canaliculata.
Highlights
Pomacea canaliculata, which belongs to the Ampullarius genus in the Ampullariidae family of Mollusca, is one of the most harmful alien invasive species in China
A total of 441,206,324 original sequences were obtained by sequencing the transcriptomes of six samples of P. canaliculata
Gene Ontology (GO) Annotation processes in the biological process category, which accounted for 56.50%, 53.59%, and 45.03%, The GOofannotation results for In
Summary
Pomacea canaliculata, which belongs to the Ampullarius genus in the Ampullariidae family of Mollusca, is one of the most harmful alien invasive species in China. These snails have been threatening agricultural production, ecological security, and public health. A large number of sequence data are available from the transcripts in organisms to generated in studies on the regulation of gene expression by using RNA-seq technology; an increasing number of scholars have applied this technology to identify drug targets through the analysis of differentially expressed genes (DEGs) after treatment with drugs. This research provides references for determining the target of pedunsaponin A in P. canaliculata at the transcription level
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