Study of the Composition and in Vivo Effect of Biologically Active Concentrates from Secondary Raw Materials of Millet

Abstract

AbstractThis work is related with the study of the properties of concentrates of biologically active substances (BAS) - polyphenols and xylooligosaccharides (XOS) from the secondary raw materials of millet (husk), and an assessment of their effect on the organism of laboratory animals. BAS was characterized by the content of the mass fraction of protein, moisture, starch, fiber, reducing substances, the qualitative and quantitative composition of phenolic substances, the fractional composition of carbohydrates, the monosaccharide composition of polysaccharides, the qualitative and quantitative compositions of XOS concentrates. The obtained BAS concentrates contain protein from 0.9%, carbohydrates - 91.50%, including XOS with prebiotic properties - 68.50% and ash 6.30%. The polyphenol concentrate is mostly represented by ferulic acid (33.47%). In the polyphenol concentrate, the yield of ferulic acid increased by 19%, gallic acid - by 2.5%, as compared to the BAS concentrate. Autopsy of laboratory animals, which were injected with 4x-chloride intraperitoneally four times during the experiment, did not reveal any visible pathological changes. Due to the action of the tested additive, the liver, with a pronounced clinical picture of acute hepatosis, which turned into a chronic form, retained its functions, which makes it possible to recommend the obtained concentrate for gastrointestinal diseases. With the introduction of epinephrine hydrochloride, which provokes degenerative changes in the structure of the heart muscle, no atomic changes were found. Thus, the possibility of practical use of millet husk as a source of antioxidants and prebiotics has been shown.KeywordsPolyphenolsBiologically active substancesSecondary raw materialsXylooligosaccharidesFerulic acidLaboratory animalsHepatosis