Abstract
Ninhydrin was found to exhibit chemiluminescence (CL) when it was oxidized with hydrogen peroxide in neutral or weakly acidic solution. Copper(II) and CoII can catalyse the CL reaction, on the basis of which ppb levels of CuII and CoII can be determined. In the presence of CuII, some amino acids enhanced the CL reaction, whereas others inhibited the CL reaction in the presence of CoII. A flow-injection system with CL detection was developed for the determination of some amino acids. The mechanism of the CL reaction is discussed.
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