STUDY OF THE CELLULOLYTIC ACTIVITY OF A COLLECTION OF "FIELD" BACTERIAL CULTURES

Abstract

Decomposition of organic substances is widespread in all types of soils and plays an important role in the carbon cycle. The decomposition of cellulose plays a special role, since cellulose is the most common polysaccharide in plant residues and can be used by new plants in the form of simpler compounds. At the same time, the rate of decomposition of plant residues in the soil takes a considerable time and largely depends on the type of soil and its microbial composition. The use of individual most active microorganisms in the composition of biological products can increase soil fertility, especially in those areas where the original composition of the microflora does not contribute to the active decomposition of plant residues. In this study, the cellulolytic activity of various strains of microorganisms isolated from the soil was studied and compared. The objects of the study were a collection of 56 strains. The study of cellulolytic activity was carried out on nutrient media containing a cellulose source using congo dye ed. The obtained results showed a different level of cellulolytic activity in the studied strains. In some cases, there was an increase in activity with a decrease in the number of bacterial cells. 18 of 56 strains demonstrated cellulose degradation only within the limits of bacterial colony growth. According to the results of the studies, 6 strains of microorganisms with the largest zone of cellulolytic activity, reaching a radius of 15 mm around the grown colony, were noted. Among the most active strains, only one was Gram-negative bacteria, while the rest were gram-positive microorganisms.